1. Fill in the patient information and collection time in 4 tubes
2.Collect each 1.0 mL in the order of gray (Nil) → green (TB1 antigen) → yellow (TB2 antigen) → purple (Mitogen), and
　the contents of the container wall (antigen) )
　10 times (or repeated for 5 seconds) conduction mixing so that) can be well mixed with
blood 

3. After collecting blood, a dedicated container is placed and stored at room temperature and

　required to arrive at the laboratory within 16 hours
4. Request for exclusive container before request